Title
Rapid Evolution of in vivo-Selected Sequences and Structures Replacing 20% of a Subviral RNA
Roles
Student co-authors:
Allison M. Murawski
Johnathan L. Nieves
Holleh F. Tajalli
Tareq Azad
Nina B. Jean-Jacques
Document Type
Article
Publication Date
9-2015
Department
Biology
Language
English
Publication Title
Virology
Abstract
The 356 nt noncoding satellite RNA C (satC) of Turnip crinkle virus (TCV) is composed of 5′ sequences from a second TCV satRNA (satD) and 3′ sequences derived from TCV. SHAPE structure mapping revealed that 76 nt in the poorly-characterized satD-derived region form an extended hairpin (H2). Pools of satC in which H2 was replaced with 76, 38, or 19 random nt were co-inoculated with TCV helper virus onto plants and satC fitness assessed using in vivo functional selection (SELEX). The most functional progeny satCs, including one as fit as wild-type, contained a 38–39 nt H2 region that adopted a hairpin structure and exhibited an increased ratio of dimeric to monomeric molecules. Some progeny of satC with H2 deleted featured a duplication of 38 nt, partially rebuilding the deletion. Therefore, H2 can be replaced by a 38–39 nt hairpin, sufficient for overall structural stability of the 5′ end of satC.
DOI
10.1016/j.virol.2015.04.002
Recommended Citation
Murawski, Allison M., Johnathan L. Nieves, Maitreyi Chattopadhyay, Megan Y. Young, Christine Szarko, Holleh F. Tajalli, Tareq Azad, Nina B. Jean-Jacques, Anne E. Simon, David B. Kushner. "Rapid Evolution of in vivo-Selected Sequences and Structures Replacing 20% of a Subviral RNA." Virology 483 (2015): 149-162.
Comments
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